Endoplasmic reticulum stress influences protective effect of ecdysterone in H2O2 induced myocardial injury
10.3969/j.issn.1000-484X.2017.11.009
- VernacularTitle:内质网应激介导蜕皮甾酮对H2O2诱导的心肌细胞损伤的保护作用
- Author:
Lei Chang GUO
1
;
Hua Feng LÜ
;
Qiang Guo WANG
Author Information
1. 新乡医学院第一附属医院心内科
- Keywords:
Endoplasmic reticulum stress;
Ecdysterone;
Cardiomyocytes;
H2O2
- From:
Chinese Journal of Immunology
2017;33(11):1642-1646
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the role of endoplasmic reticulum stress(ERS)on ecdysterone(EDS)influenced protective effect in H2O2induced myocardial injury.Methods: The H9c2 cells were randomly divided into control group(normal cells),H2O2 groups(the cells treated with H2O2at concentrations of 1×10-3,1×10-4,1×10-5mol/L,respectively);EDS group(the cells were exposed to H2O2and treated with EDS at concentrations of 25,50,100 μmol/L respectively).The cell activity and apoptosis were measured by MTT assay and flow cytometry,respectively.The protein levels of Bcl-2,Bax,cleaved-caspase-3,caspase-4,caspase-7, caspase-12,GRP78 and CHOP were deter mined by Western blot.The MDA content and SOD activity were detected by the MDA and SOD detection kits.Results:The cell activity was decreased,cell apoptosis was increased,the content of MDA was elevated,the activity of SOD was inhibited and the protein expression of GRP78 and CHOP were increased in H2O2group as compared with control group, which were all significantly reversed by EDS treatment(P<0.05).Conclusion: Ecdysterone exhibits a protective effect on the cardiomyocytes with H2O2induced injury by inhibiting endoplasmic reticulum stress.
