Effects of Chinese herbal compound "Jisuikang" on phagocytosis of microglia and regeneration of injured neurons in co-culture system
10.3969/j.issn.1000-484X.2017.11.011
- VernacularTitle:脊髓康对共培养体系中小胶质细胞吞噬及损伤神经元再生的影响
- Author:
Lan Ya PAN
1
;
Yang GUO
;
Yun Long ZHOU
;
Chao Wen YUAN
;
Yong MA
;
Cheng Gui HUANG
Author Information
1. 南京中医药大学骨伤研究所
- Keywords:
Herbal compound;
Jisuikang;
Primary microglia;
Primary neurons;
Co-culture;
Phagocytosis;
Regeneration of neu-rons
- From:
Chinese Journal of Immunology
2017;33(11):1652-1657
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Chinese herbal compound'Jisuikang'on the phagocytosis of microglia and the regeneration of injured neurons in co-culture system.Methods: Prepared drug serum of 'Jisuikang′ and isolated and identified the primary neuron and microglia.The neuron cells were induced apoptosis by glutamic acid and the microglia cells were predisposed by drug serum of'Jisuikang'.Then,the co-culture system of injured neurons and microglia cells was established.24 h and 96 h after co-culture,engulfment of neuron debris by microglia cells and regeneration of injured neurons were observed by immunofluorescence double labeling method.Results: 24 h after co-culture,middle and high dose of'Jisuikang' showed greater phagocytic percentage and phagocytic index than that of control.In comparison of LPS,high dose of'Jisuikang' showed no significant difference.96 h after co-culture,first grade of neuritis of middle and high dose of'Jisuikang' were more than that of control,and there were no significant difference in comparison of LPS.Neuritis' mean length per cell of middle and high dose of'Jisuikang' were larger than that of con-trol.Neuritis' mean length per cell of high dose of'Jisuikang' showed significant difference in comparison of LPS.Conclusion:Traditional Chinese medicine compound'Jisuikang'may enhance engulfment of neuron debris by microglia to improve local microenvi-ronment,which promote the repair and regeneration of injured neurons.
