Effect of bone marrow mesenchymal stem cells on cognitive function after traumatic brain injury in rats
10.3969/j.issn.1000-484X.2017.11.014
- VernacularTitle:骨髓间充质干细胞对大鼠脑创伤后认知功能的影响
- Author:
Jie Ying WANG
1
;
Jing WANG
;
Xin SHI
Author Information
1. 承德医学院
- Keywords:
BMSCs;
Traumatic brain injury;
HE;
Morris water maze
- From:
Chinese Journal of Immunology
2017;33(11):1668-1672
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of bone marrow mesenchymal stem cells transplantation on the cognitive function in hippocampus after traumatic brain injury in rats.Methods: BMSCs were isolated and cultured from male 3-week-old SD rats in vitro.60 female SD rats were randomly divided into 3 groups(n=20),control group,model group,BMSCs treatment group.After the es-tablishment of brain trauma model of the model 1,7,14,28 d,BMSCs treatment group was given BMSCs(1×106ml-1)intervention.In addition,20 rats were tested by water maze,and each group was divided into four points:6,7,8 9 d after injury.The morphological changes of brain tissue was detected by HE staining;the water content of brain tissue was detected by dry wet method;Morris detection method,detection of spatial learning and memory ability in rats;nerve motor function score, neural function analysis detection;field test,detection of spatial learning and memory ability in rats.Results: The morphological changes of brain were observed by HE staining;the water content of brain tissue was detected by wet and dry gravity;the water maze test was conducted to examine the spatial learning ability,the motor function score,and the field experiment.The results of wet and dry gravity test showed that compared with the control group,the model group was significantly higher than the control group(P< 0.05),the water content of the treatment group at each time was significantly lower than the model group;the results of water maze test showed that the latency of safe island was significantly increased in the model group on the eighth and ninth day after injury,compared with the control group,and the difference was statistically significant(P<0.05).In the treatment group,the safe island latency was significantly shorter in the eighth day and ninth day after injury than in the control group;the neurological score results showed that compared with control group,model group,rats in the hind limb grasping reflex,vibrissae evoked forelimb positioning and lateral pad step tests were not normal performance,resulting in functional score decreased with statistical significance(P<0.05).The functional scores of the treatment group and the model group were significantly higher than those of the model group.Conclusion:Through the behavioral related tests,the data show that BMSCs can restore the neurological function to a certain extent after traumatic brain injury in rats.
