Campylobacter jejuni AhpC protein B cell antigen epitope prediction and antigenic analysis

VernacularTitle:空肠弯曲菌AhpC蛋白B细胞抗原表位预测及抗原性分析
Author: hao Hao CHEN ¹ ; sheng Xu LI ; ming Fang GUO ; xing Ming DING ; Ye HU ; qiang Hong LOU
Author Information

1. 金华职业技术学院医学院分子生物学实验室
Keywords: Campylobacter jejuni; epitope; antigen; alkyl hydroperoxide reductase
From: Chinese Journal of Zoonoses 2017;33(10):868-871
CountryChina
Language:Chinese
Abstract: To provide evidence for Campylobacter jejuni (C.jejuni) vaccine research,the B cell epitope of AhpC protein were predicted,and the antigenicity was analyzed.AhpC was found locating in outer membrane of C.jejuni without transmem brane structure and no signal peptide by bioinformatics software TMHMM Server V2.0,SignalP 4.1.There were seven B-cell linear epitopes in AhpC predicted by IEDB.Then,the AhpC protein and chemically synthesized antigenic epitopes of C.jejuni were used as antigens,and the AhpC antibody of C.jejuni was used as the primary antibody,the antigens of predominant linear B cell epitopes were detected by ELISA and dot blot.Results showed that one epitope of B cell epitopes (AhpC4-16) was able to recognize the antibodies of C.jejuni AhpC and had strong antigenicity,indicating that could be used in the follow-up vaccine research.