Sequence analysis and structure prediction of the methyltransferase of duck Tembusu virus
10.3969/j.issn.1002-2694.2017.10.005
- VernacularTitle:鸭坦布苏病毒甲基转移酶的序列分析和结构预测
- Author:
dong Xue WU
1
;
tian Yue ZHANG
;
yong Ren JIA
Author Information
1. 四川农业大学禽病防治研究中心
- Keywords:
duck Tembusu virus;
methyltransferase;
sequence analysis;
structure prediction
- From:
Chinese Journal of Zoonoses
2017;33(10):877-881
- CountryChina
- Language:Chinese
-
Abstract:
In order to determine the amino acid sequence of functionthat play a role of methyltransferase (MTase) activity in duck Tembusu virus (DTMUV),we have made sequence analysis and structure prediction of the MTase in DTMUV by bioin formatics methods,as well as using the sequence and structure of MTase in other flaviviruses which have been reported.The bioinformatical analysis results showed that the MTase and the MTase of other three kinds of flaviviruses had the higher homology in nucleotide sequence and amino acid sequence,for 64.76％,64.55％,67.33％ and 74.71％,68.45％,75.15％ respectively,and the same basic structural characteristics which contained SAM binding site and had a typical of 4 alpha screw and 7 beta folding.At the same time,there were classical conservative sites K-D-K-E of flavivirus MTase in DTMUV MTase sequence.In conclusion,they imply that the MTase of DTMUV might belong to MTase family of flaviviruses.
