Content Determination of Geniposide in Biyuanshu Oral Liquid by Micellar Electrokinetic Capillary Chromatography
10.3969/j.issn.1005-5304.2017.12.017
- VernacularTitle:胶束电动毛细管色谱法测定鼻渊舒口服液中栀子苷含量
- Author:
yan Xiao LIAO
1
;
lu Chen WANG
;
hong Jing PENG
;
qing Yu YANG
;
Xiu ZHONG
;
cai Shu LIANG
;
xiu Yu XIAO
Author Information
1. 武汉大学药学院
- Keywords:
Biyuanshu Oral Liquid;
geniposide;
micellar electrokinetic capillary chromatography;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(12):68-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a micellar electrokinetic capillary chromatography method for content determination of geniposide in Biyuanshu Oral Liquid. Methods Acetaminophen was used as an internal standard, and the separation was performed on an uncoated fused silica capillary of 52 cm × 50 μm ID (42 cm effective length) with the separation voltage of 25.0 kV. The running buffer contained 50 mmol/L borax, 100 mmol/L sodium dodecyl sulfate and 15％ acetonitrile (pH=10). The sample was injected by pressure (10 s, 0.5 psi) and detected at 238 nm. Results Geniposide was in good linearity range of 15.02–320.48 μg/mL (r=0.9995). The repeatability (low, medium and high concentration of samples) and intermediate precision assays gave satisfactory RSD values of less than 1.77％and 2.01％, respectively. The average recovery of geniposide in Biyuanshu Oral Liquid was 97.50％ and the RSD was 4.43％. The contents of geniposide determined by micellar electrokinetic capillary chromatography were in accordance with the results of HPLC analysis. Conclusion The method is simple, fast, accurate and precise, which can be used for the content determination of geniposide in Biyuanshu Oral Liquid.
