Clinical significance of serum 14-3-3η protein and auto-antibodies detection in rheumatoid arthritis
10.3969/j.issn.1000-484X.2017.11.019
- VernacularTitle:类风湿关节炎患者检测血清14-3-3η蛋白和自身抗体的临床意义
- Author:
Ting Ting ZENG
1
;
Ming Li TAN
;
Juan Juan CHEN
;
Qing Yong JIANG
;
Yang WU
;
Jian Yong TIAN
;
Lin Jian YU
;
Hua LI
;
Wang Xin DUAN
Author Information
1. 江西省医学检验重点实验室
- Keywords:
Rheumatoid arthritis;
14-3-3η protein;
Anti-CCP;
Anti-Sa;
Anti-RA33;
Rheumatoid factor
- From:
Chinese Journal of Immunology
2017;33(11):1689-1693
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical value of detecting serum 14-3-3η and auto-antibodies in rheumatoid arthritis (RA),and compare their performance in RA diagnosis.Methods: Serum samples of 134 RA patients,90 non-RA inflammatory arthropathy patients,70 of whom with osteoarthritis(OA)and 20 with ankylosing spondylitis(AS),and 40 healthy controls from the second affiliated hospital of Nanchang University were collected.Concentrations of 14-3-3η,anti-CCP,anti-RA33,anti-Sa were detected with enzyme linked immunosorbent assay(ELISA),with RF detected by immunonephelometry.Diagnostic utilities of them for RA were evaluated and compared then.Results:① Serum levels of 14-3-3η,anti-CCP,anti-RA33,anti-Sa and RF were significantly higher in patients with RA than non-RA inflammatory arthropathy patients and healthy controls,the differences between groups were statistically significant;② ROC curves were conducted according to the serum levels detected.The AUC of 14-3-3η,anti-CCP,anti-RA33,anti-Sa and RF were 0.831(95% CI:0.782-0.881),0.852(95% CI:0.802-0.901),0.615(95% CI:0.546-0.684),0.706(95% CI:0.643-0.770)and 0.739(95% CI:0.676-0.802)respectively,with P values<0.01.Among all index,only anti-CCP and 14-3-3η were of moderate diagnostic value,at the threshold of 24.10 U/ml and 2.59 ng/ml individually;③anti-Sa was of highest specificity and RF was of highest sensitivity among all indexes detected;the specificity of 14-3-3η was merely moderately inferior to anti-Sa and anti-RA33,but its sensitivity was superior to them both.Conclusion:Serume14-3-3η,anti-CCP,anti-RA33,anti-Sa and RF levels increased remarkably in patients with RA,and contributed to RA diagnosis.Meanwhile,14-3-3η was advantageous,to some extent,in the sensitivity and specificity over auto-antibodies,and can be utilized as a reference index in diagnosing RA.
