Aminoglycoside resistance genes of Acinetobacter baumannii
10.3969/j.issn.1002-2694.2017.10.012
- VernacularTitle:48株鲍曼不动杆菌氨基糖苷类抗生素耐药基因检测
- Author:
Pu GUO
1
;
Yan QIAO
;
Jing LI
Author Information
1. 蚌埠医学院第一附属医院检验科
- Keywords:
Acinetobacter baumanni;
aminoglycosides antibiotics;
drug resistance
- From:
Chinese Journal of Zoonoses
2017;33(10):912-915
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the distribution of the resistance genes of Acinetobacter baumannii to aminoglycoside,48 strains of extensively drug resistant Acinetobacter baumannii were collected from the First Affiliated Hospital of Bengbu Medical College from January to December,2015.The drug sensitivity test and identification were performed by VITEK 2 compact automatic microorganism instrument.Twelve aminoglycosides modifying enzymes,three 16SrRNA methylase genes and efflux pump abeB gene were detected from these isolates by PCR.Results showed that among these experimental 16 genes,aac(6')-Ⅰb gene was detected from 19 of 48 isolates (39.6％),both armA and adeB genes were 43 (89.6％),ant(3")-Ⅰa gene was from 5 (10.4％),while the other genes were not found.And more than two gene types were amplified from 39 of 48 strains (81.3％).In conclusion,the aac(6')-Ⅰb,armA gene and efflux pump adeB may play a key role in drug resistance to aminoglycosides antibiot ics of Acinetobacter Baumanni in our hospital.
