Alleviation of aortic vascular dysfunction in STZ/HFD-induced type 2 diabetic rats by nFGF1
10.3969/j.issn.1000-4718.2017.11.004
- VernacularTitle:成纤维细胞生长因子1非促分裂突变体对2型糖尿病大鼠血管功能的保护作用
- Author:
Yan NAN
1
;
Na LI
;
Yu DONG
;
cheng Peng QIU
;
sheng Zhi HU
;
zhou Chang CAI
;
guo Ling KONG
;
le Zheng HE
;
Lei YING
;
Yang WANG
Author Information
1. 温州医科大学附属第二医院育英儿童医院新生儿科
- Keywords:
Type 2 diabetes;
Fibroblast growth factor 1;
Inflammation;
Oxidative stress
- From:
Chinese Journal of Pathophysiology
2017;33(11):1945-1950
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the protective effect of non-mitogenic fibroblast growth factor 1 (nFGF1) on the aortic vascular function in streptozotocin (STZ)/high-fat diet (HFD)-induced type 2 diabetic rats and its underlying mechanisms. METHODS:Five-week-old male SD rats (n=30) were randomly divided into 3 groups (n=10 in each group),including normal control group,type 2 diabetic group and nFGF1 treatment group(type 2 diabetic rats were intra-peritoneally injected with 0.5 mg/kg nFGF1 every other day for 4 weeks). After the rats were sacrificed, blood glucose, cholesterol and triglyceride levels,aorta diastolic function and superoxide dismutase(SOD) level in the aorta of each group were measured. Besides,the protein levels of cyclooxygenase-2(COX-2),phosphorylated extracellular signal-regulated ki-nase (p-ERK) and endothelial nitric oxide synthase (eNOS) in the aorta were determined by Western blot. RESULTS:nFGF1 markedly lowered blood glucose, cholesterol and triglyceride levels, enhanced aorta SOD activity and upregulated protein level of eNOS in the type 2 diabetic rats. Furthermore,the increased protein levels of COX-2 and p-ERK in the type 2 diabetic rats were largely abrogated by nFGF1. CONCLUSION:nFGF1 effectively attenuates aortic vascular dysfunction in the type 2 diabetic rats,which may be associated with decreasing blood glucose,cholesterol and triglyceride levels,re-ducing inflammation and oxidative stress response,and activating eNOS signaling pathway.
