Effects of aspirin on apoptosis of homologous nasopharyngeal carcinoma cells with different radioresistance
10.3969/j.issn.1000-4718.2017.11.010
- VernacularTitle:阿司匹林对同源不同辐射抗拒能力鼻咽癌细胞的凋亡诱导作用
- Author:
fei An LI
1
;
dan Hai LUO
;
Yu CHEN
;
jin Bo SU
;
hui Dong WANG
;
ling Hui YANG
Author Information
1. 中山大学中山医学院病理生理教研室
- Keywords:
Nasopharyngeal neoplasms;
Radioresistance;
Aspirin;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2017;33(11):1980-1986
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of aspirin on the apoptosis of nasopharyngeal carcinoma cell lines CNE2R and CNE2 with different radioresistance and its potential mechanism. METHODS:The effects of aspirin on the cell viability, apoptosis, and protein levels of procaspase-3, cleaved caspase-3, procaspase-9, procaspase-12, PARP and cleaved PARP,PI3K p110α,Akt,Bcl-2,Bax and p27 in the CNE2R cells and CNE2 cells were detected by the methods of MTT assay,flow cytometry and Western blot. RESULTS:Aspirin inhibited the viability of homologous nasopharyngeal carcinoma cell lines CNE2 and CNE2R(with the IC50to CNE2 cells of 6.18,3.92 and 3.06 mmol/L for 24 h,48 h and 72 h,respectively;and with the IC50to CNE2R cells of 7.05,3.90 and 2.20 mmol /L for 24 h,48 h and 72 h,respec-tively). After treated with aspirin for 48 h, the apoptotic rate of CNE2R cells was higher than that of CNE2 cells (P<0.05). After treated with aspirin for 48 h,the protein levels of procaspase-3,procaspase-9,procaspase-12 and PARP were decreased,the protein levels of cleaved caspase-3,cleaved PARP and p27 were increased,and the protein levels of PI3K p110α,Akt and Bcl-2/Bax were decreased. CONCLUSION:Aspirin inhibits the viability of homologous nasopharyngeal carcinoma cell lines CNE2R and CNE2 with different radioresistance. Aspirin also induces the apoptosis of CNE2 and CNE2R cells,which is more effective in CNE2R cells. The underlying mechanisms may be involved in affecting PI3K/Akt signaling pathway,Bcl-2/Bax and p27 expression.
