Effect of CKLF1-C19 polypeptide on differentiation of human lung fibro-blast into myofibroblast induced by TGF-β
10.3969/j.issn.1000-4718.2017.11.020
- VernacularTitle:CKLF1-C19多肽对TGF-β诱导原代人肺成纤维细胞向肌成纤维细胞转化的干预作用
- Author:
Hang LONG
1
;
xia Ya TAN
;
Yuan QIU
;
qing Yan SHI
;
sheng Yan WANG
;
hong Jie HUANG
;
liang Wen ZHOU
Author Information
1. 广州医科大学附属第一医院广州呼吸疾病研究所
- Keywords:
CKLF1-C19 polypeptide;
Lung fibroblasts;
Myofibroblasts;
Airway remodeling
- From:
Chinese Journal of Pathophysiology
2017;33(11):2047-2052
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of CKLF1-C19 polypeptide (C19) on differentiation of human lung fibroblast (LFB) into myofibroblast (MFB) induced by TGF-β. METHODS:LFBs were cultured and identified. LFBs were treated with TGF-β(5 μg/L) to establish the cell model of LFB differentiate into MFB. The LFBs were divided into 6 experimental groups including control group,TGF-β group,and TGF-β plus different doses(1,0.1,0.01,0.001 mg/L) C19 groups. The cell morphology,cell proliferation rate, and the expression of α smooth muscle actin (α-SMA) and collagen Ⅰ were observed. RESULTS:Human primary LFB was successfully cultured and was confirmed by the method of immunofluorescence. TGF-β at 5 μg/L induced proliferation and differentiation of LFB. The mRNA levels of α-SMA and collagen Ⅰ in TGF-β group were higher than that in control group(P<0.05).The cell proliferation rates,mRNA levels of α-SMA and collagen Ⅰ, and the protein expression of α-SMA in 0.01 mg/L+TGF-β group and 0.001 mg/L+TGF-β group were markedly lower than those in TGF-β group(P<0.05). CONCLUSION:C19 at 0.01 mg/L and 0.001 mg/L effectively inhibits differentiation of LFB into MFB induced by TGF-β, thus inhibiting the process of airway remodeling and fibrosis to some extent.
