Erythropoietin inhibits eryptosis induced by reactive oxygen species
10.3969/j.issn.1000-4718.2017.11.026
- VernacularTitle:红细胞生成素抑制活性氧诱导的红细胞衰亡
- Author:
Yun SUN
1
;
Gang LIU
;
li Ya JIANG
;
Bin ZHANG
;
Xuan ZHAO
;
gang Xue LI
Author Information
1. 山东大学第二医院肾内科
- Keywords:
Hydrogen peroxide;
Eryptosis;
Erythropoietin;
Reactive oxygen species;
Intracellular calcium ion concentration
- From:
Chinese Journal of Pathophysiology
2017;33(11):2084-2089,2094
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the influence of erythropoietin (EPO) on eryptosis and production of reactive oxygen species (ROS) in erythrocytes under stimulation of hydrogen peroxide (H2O2),.and to explore its related mecha-nism. METHODS:The erythrocyte suspension (1%) was cultured in vitro and divided into 3 groups:control group (C group,the culture medium was PBS),H2O2group (H group,the culture medium was PBS containing H2O2at final con-centration of 100 μmol/L) and EPO group (E group,the culture medium was PBS containing H2O2at final concentration of 100 μmol/L and EPO at final concentration of 2×104U/L). The erythrocytes were collected at 24 h and 60 h. The eryptosis was detected by flow cytometry with Annexin V staining. The production of ROS and intracellular calcium ion con-centration (Ca2+]i) were also analyzed by flow cytometry. RESULTS:The eryptosis in C group was increased as the in-cubating time extended. The eryptosis in H group was higher than that in C group (P<0.01),while that in E group was lower than that in H group(P<0.01). Meanwhile,ROS production andCa2+]iwere higher in H group than those in C group (P<0.01), but those were lower in E group than those in H group (P<0.05 or P<0.01). CONCLUSION:EPO inhibits eryptosis induced by H2O2and its mechanism may be related to antioxidant effect and change of Ca2+]i.
