Knockdown of growth hormone receptor prevents growth hormone in-duced inflammatory cytokine production in 3T3-L1 adipocytes
10.3969/j.issn.1000-4718.2017.11.027
- VernacularTitle:干扰3 T3-L1脂肪细胞生长激素受体对生长激素诱导的细胞炎症因子表达和分泌的影响
- Author:
lin Hui QIAN
1
;
Liang HUANG
;
Ru CHAO
;
Fan ZHOU
;
lin Shun QU
;
Chi ZHANG
Author Information
1. 南华大学心血管病研究所
- Keywords:
Chronic low-grade inflammation;
Adipocytes;
Growth hormone;
Growth hormone receptor;
In-flammatory cytokines
- From:
Chinese Journal of Pathophysiology
2017;33(11):2090-2094
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of growth hormone receptor(GHR) knockdown on nuclear factor-κB (NF-κB) activity and inflammatory cytokine production stimulated by growth hormone (GH) in 3T3-L1 adipocytes. METHODS:The specific siRNA for GHR was transfected into 3T3-L1 adipocytes to silence GHR expressions. The effects of GH on NF-κB activation and inflammatory cytokine production in 3T3-L1 adipocytes transfected with siRNA-GHR or siRNA-control were measured by dual-luciferase system analysis,real-time RT-PCR and ELISA. RESULTS:The protein expression of GHR was diminished after transfection with GHR specific siRNA. Dual-luciferase reporter system analysis re-vealed that GHR knockdown resulted in attenuation of GH-stimulated NF-κB activation in the 3T3-L1 adipocytes. GHR knockdown ameliorated the GH-induced production of inflammatory cytokines TNF-α,IL-1β,IL-6,MCP-1 and MIP-1α in the 3T3-L1 adipocytes. CONCLUSION:Knockdown of GHR might be efficacious to prevent GH-induced inflammatory re-sponses in the 3T3-L1 adipocytes.
