Effects of β-carotene on expression of cell tight junction protein of IPEC-J2 with LPS-stimulated
10.3969/j.issn.1000-484X.2017.11.003
- VernacularTitle:β-胡萝卜素对脂多糖刺激的IPEC-J2细胞紧密连接蛋白表达的影响
- Author:
Nan Ruo LI
1
;
Pan HONG
;
Ying Wu LANG
;
Xin ZHENG
Author Information
1. 吉林农业大学动物科学技术学院
- Keywords:
β-carotene;
IPEC-J2 cell;
LPS;
Tight junction protein
- From:
Chinese Journal of Immunology
2017;33(11):1611-1615
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of β-carotene anti-inflammatory on intestinal epithelial cells.Methods:The piglet jejunum epithelium(IPEC-J2)cell line was used as an cell model.The cells were divided into 4 groups[control group,β-carotene group,β-carotene pre-protective group and Lipopoly-saccharide(LPS)group].The control group was not treated,β-carotene group and β-carotene pre-protective group were pretreated with β-carotene.Lipopoly-saccharide(LPS)and β-carotene pre-protective groups were stimulated with LPS.The cell viability was detected by MTT.Western blot was performed to detect the expression of Occludin,Claudin4 and ZO-1 tight junction proteins.Results:The expression of IPEC-J2 cell tight junction protein in LPS group were significantly lower than that of the control group(P<0.05).The expression of tight junction protein in β-carotene pre-protective group was significantly higher than that in LPS group(P<0.05).Conclusion:Increasing the expression of tight junction proteins may be one of the ways that anti-inflammatory effect of β-carotene in jejunum epithelial cells.
