Effects on phenotype and function of mouse CIK cells treated with carboxylated single-walled carbon nanotubes
10.3969/j.issn.1000-484X.2017.11.004
- VernacularTitle:羧基化单壁碳纳米管对小鼠CIK细胞表型及功能的影响研究
- Author:
Ze Ying WANG
1
;
Lan Qiao WANG
;
Chao DU
;
Qing Qing CAO
;
Yu FU
Author Information
1. 河北科技大学生物科学与工程学院
- Keywords:
Carboxylated single-walled carbon nanotubes;
CIK cells;
Tumor immunity;
Immunophenotype
- From:
Chinese Journal of Immunology
2017;33(11):1616-1620,1625
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect on the phenotype and function of CIK cells induced from splenic lymphocyte in mice treated with carboxylated single-walled carbon nanotubes.Methods:CIK cells were treated with carboxylated single-walled carbon nanotubes at different concentrations.Cell growth status was observed using inverted fluorescence microscopy.The killing effect was de-termined by MTS method.Immunophenotype was analyzed by flow cytometry.Results: With the increase of the effect target ratio,the killer rate of CIK was gradually enhanced and the optimal effect target ratio was 20:1.With the increase of the dose of carboxylated single-walled carbon nanotubes,the proportion of Treg cells decreased.When CIK cells were treated with 0.5 μg/ml carboxylated single-walled carbon nanotubes(CNTs),the proportion of CD3+CD4+and CD3+CD8+was significantly higher than that of the control group(P<0.05),and the killing effects of CIK anchieved best results to B16 cells,H22 cells and RM-1cells.Conclusion: The carboxylated single-walled carbon nanotubes enhanced the ability of CIK cells to kill tumor cells,significantly which provide has potential value in tumor drug development.
