Effect of Dai medicine Yapan on TGF-β1/Smads signaling transduction pathway in unilateral ureteral obstruction rats
10.3969/j.issn.1671-8348.2017.31.002
- VernacularTitle:傣药雅盼对单侧输尿管梗阻大鼠TGF-β1/Smads信号转导通路的影响
- Author:
Jian WANG
1
;
Hongling YUAN
Author Information
1. 云南省第一人民医院肾内科
- Keywords:
Dai medician Yapan;
renal interstitial fibrosis;
transforming growth factor-β1;
unilateral ureteral obstruction;
rats
- From:
Chongqing Medicine
2017;46(31):4325-4328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Dai medicine Yapan on TGF-β1/Smads signaling transduction pathway in unilateral ureteral obstruction (UUO) rats,and to explore its possible mechanism in rat renal interstitial fibrosis inhibitory effect.Methods The UUO rat model was established by conducting the unilateral ureteral obstruction(UUO) in 120 male rats.Normal saline gavage or medication were performed for 28 d and serum was collected for detecting BUN and Scr.Then the rats were killed for taking the renal tissue and conducting HE and Masson staining.The expression of TGF-β1,Smad2 and Smad7 in kidney tissues were detected by using the immunohistochemical method.The mRNA expression was detected by RT-PCR;the protein expression was detected by Western blot.Results Various Yapan doses groups could reduce the level of serum BUN and Scr in experimental rats,alleviated renal tubular interstitial inflammatory cells and renal interstitial fibrosis degree,meanwhile up-regulated the expression of TGF-β1,Smad2 mRNA and protein (P＜0.05),while down-regulated the expression of Smad7 mRNA and protein (P＜ 0.05).Conclusion Different concentrations groups of Dai medician Yapan may inhibit Smad2 mRNA expression in some extent,upregulates Smad7 mRNA expression,inhibits the expression of TGF-β1 in kidney tissues through interfering TGF-β1/Smads signaling transduction pathway,thus obtains a therapeutic effect for inhibiting kidney interstitial fibrosis in UUO rat.
