Effects of lycium barbarum polysaccharide on UV-mediated DNA strand breakage damage in HSF cells
10.3760/cma.j.issn.1671-0290.2017.05.015
- VernacularTitle:枸杞多糖对紫外线致人皮肤成纤维细胞DNA链断裂损伤的影响
- Author:
Liqian PENG
1
;
Zhenjie LI
;
Erting ZHANG
;
Qing LIU
;
Na JIANG
;
Huaping LI
;
Bihua LIANG
;
Runxiang LI
;
Huilan ZHU
Author Information
1. 510095,广州医科大学皮肤病研究所
- Keywords:
Lycium barbarum polysaccharide;
Human Skin Fibroblasts;
ROS;
DNA Damage
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2017;23(5):336-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of lycium barbarum polysaccharide (LBP) on DNA damage of HSF cells induced by UV.Methods We established the model of UV induced photo damage in HSF cells.We detected the viability of HSF cells by using MTT colorimetry.The UV absorption spectrum of LBP was also measured by UV spectrophotometer.The level of ROS was detected by DCFH-DA fluorescent probe method.Comet assay was employed to evaluate the DNA strand breakage damage.Results When the concentration of LBP was less than or equal to 300μg/ml,there was no significant effect on the proliferation of HSF cells (P＞0.05).When the concentration was more than 300 μg/ml,it could inhibit the cell proliferative activities (P＜0.05).Compared to the UV groups,UV+LBP groups can respectively improve the cell proliferation activity (P＜0.05).The absorbance was slight range 280 from 400 nm.Compared with the UV group,the relative fluorescence intensity and the migration distance of UV+ LBP groups were significantly decreased (P＜0.05).Conclusions Lycium barbarum polysaccharide can effectively inhibit the proliferation activity and protect the breakage of DNA strand induced by UV,which is probably due to its action of removing free radicals.
