Identification of Differentially Expressed Genes from Serum Deprived p388D1 Cells.
- Author:
Su Young KIM
;
Sang Ho KIM
;
Sug Hyung LEE
;
Nam Jin YOO
;
Jung Young LEE
;
Choo Soung KIM
- Publication Type:Original Article
- Keywords:
Growth arrest;
Differential display;
Gene expression
- MeSH:
Amino Acid Sequence;
Cell Cycle;
Clone Cells;
Gene Expression;
Ribonucleases;
Starvation
- From:Korean Journal of Pathology
1998;32(7):488-493
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This experiment is designed to find differentially expressed genes in p388D1 cells that are specific for the serum deprived state. Serum starvation induces cells to enter the quiscent state in the cell cycle and is used to arrest cell growth or synchronize the cell cycle. Differential display and ribonuclease protection assay were used to identify quantitative change in gene expression. Nineteen genes that showed a differential expression in the differential display were cloned and 7 clones were verified by a ribonuclease protection assay. Among the 7 clones clone-16 showed same expression pattern in comparison with the differential display. Deduced amino acid sequences of clone-16 had N-glycosylation motif and seems to be a secretory protein. Getting a full sequence of clone-16 is critical for the characterization of it.
