Paeoniflorin alleviates TNF-α induced intestinal barrier dysfunction by inhibition of MLCK
10.3969/j.issn.1001-1978.2017.11.013
- VernacularTitle:芍药苷抑制肌球蛋白轻链激酶缓解肠上皮细胞屏障功能紊乱
- Author:
Liang WANG
1
;
Fang YANG
;
mei Si HUI
;
juan Zi ZHOU
;
Jun CHEN
;
wei Hong ZHAN
;
peng Da CHEN
;
yu Jing WANG
Author Information
1. 大连医科大学实验动物中心
- Keywords:
ulcerative colitis;
paeoniflorin;
tumor necrosis factor alpha;
Caco-2 cells;
intestinal epithelial barrier;
myosin light chain kinase
- From:
Chinese Pharmacological Bulletin
2017;33(11):1541-1545
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of paeoniflorin on TNF-α induced intestinal epithelial barrier dysfunction and its mechanisms.Methods The Caco-2 cells were cultured and the MTF assay was used to determine the effects of the paeoniflorin on Caco-2 cell activity.The Caco-2 cell intestinal epithelial barrier dysfunciton model was established through incubation of cells with TNF-α.The effects of paeoniflorin on intestinal epithelial barrier dysfunciton were studied.Results The transmembrane resistance in Caco-2 epithelial barrier was significantly reduced by TNF-α incubation;MLCK significantly increased,while tight junction protein occludin and ZO-1 significantly decreased by TNF-α.These changes were significantly reversed by paeoniflorin,which reduced MLCK expression and enhanced expression of occludin and ZO-1.The protective effects against epithelial barrier dysfunction could be abrogated by small interfering RNA(siRNA) of MLCK.Conclusions Paeoniflorin alleviates the epithelial barrier dysfunction induced by TNF-αthrough down-regulation of MLCK and enhancement of tight junction protein occludin and ZO-1.This study supplies a potential candidate drug for the clinical treatment of inflammatory bowel diseases.
