Effects of active fraction from Pegasus laternarius Cuvier on proliferation and apoptosis of human cervical cancer HeLa cells
10.3969/j.issn.1001-1978.2017.11.014
- VernacularTitle:海蛾活性部位抑制人宫颈癌HeLa细胞增殖及诱导凋亡作用研究
- Author:
Feng LI
1
;
feng Shi PEI
;
ai Xian SHI
Author Information
1. 福州大学药物生物技术与工程研究所
- Keywords:
Pegasus laternarius Cuvier;
HeLa cells;
bioassay-guided method;
apoptosis-inducing;
flow cytometry;
Bcl-2;
caspase-3
- From:
Chinese Pharmacological Bulletin
2017;33(11):1546-1552
- CountryChina
- Language:Chinese
-
Abstract:
Aim To isolate HeLa cell proliferation-inhibitory active fraction from Pegasus laternarius Cuvier and explore its potential apoptosis-inducing mechanism.Methods To obtain the active fraction,the ethanol extract of Pegasus laternarius Cuvier was chromatographed by silica gel and sephadex LH-20 columns;MTT assay was used to evaluate the proliferation-inhibitory ability of active fraction on HeLa cells;AO/EB,PI and Annexin V-FITC/PI fluorescent staining flow cytometry were used to evaluate its apoptosis-inducing ability;the possible mechanism was investigated by analyzing the enzyme activity of caspase-3 and the protein expression of apoptosis-related genes in tumor cells.Results A fraction of C22 with high HeLa proliferation-inhibitory activity was isolated,with a yield of 0.73 ‰ and an IC50 of 36.3 mg · L-1;fraction C22 could increase the proportion of cells in sub-G0/G1 phase,phosphatidylserine eversion and other typical cell apoptosis in a dose-dependent manner;fraction C22 could down-regulate the expression of Bcl-2 and increase the enzyme of caspase-3 in HeLa cells.Conclusions The active fraction C22 from Pegasus laternarius Cuvier can inhibit the proliferation of HeLa cells by inducing apoptosis.The effect of inducing apoptosis may be conducted through mediating the mitochondrial Bcl2/caspase pathway.
