Curcumin inhibits preadipocyte differentiation by reducing CREB transcriptional activity
10.3969/j.issn.1001-1978.2017.11.017
- VernacularTitle:姜黄素通过减少CREB转录活性抑制前脂肪细胞分化
- Author:
Jie CHEN
1
;
ran Yi LIU
Author Information
1. 武汉职业技术学院生物工程学院
- Keywords:
curcumin;
cAMP-response element binding protein;
preadipocytes;
cell differentiation;
CCAAT/enhancer binding protein β;
CCAAT/enhancer binding protein α;
peroxisome proliferator activated receptor γ
- From:
Chinese Pharmacological Bulletin
2017;33(11):1564-1569
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of curcumin on differentiation of 3T3-L1 preadipocytes and its mechanism.Methods 3T3-L1 preadipocytes were treated with curcumin at different concentrations (10,20,40 μmol · L-1) and cell differentiation was synergistically induced.The proliferation of 3T3-L1 preadipocytes was detected by MTT assay.The lipid accumulation was detected by oil red O staining method,and the levels of CREB,C/EBPβ,C/EBPα,PPARγmRNA and protein in 3T3-L1 preadipocytes were detected by qRT-PCR and Western blot.Results MTT results showed that curcumin had a significant inhibitory effect on proliferation of 3T3-L1 preadipocytes in a dose-and time-dependent manner.Oil red O staining showed that curcumin inhibited the differentiation of 3T3-L1 preadipocytes in a dose-dependent manner.When 0,20,40 μmol · L-1 curcumin treatment of 3T3-L1 preadipocytes were performed,the levels of p-CREB,C/EBPβ,C/EBPα,PPARγ mRNA and protein significantly decreased with the increasing dose of curcumin (P ＜ 0.05).Conclusions Curcumin can effectively inhibit the proliferation and differentiation of 3T3-L1 preadipocytes.The mechanism may be that curcumin inhibits CREB transcriptional activity and inhibits the expression of C/EBPβ,C/EBPα and PPARγ.
