Analysis of components in plasma from effective fractions of Inula cappa based on UHPLC/Q-TOF/MS
10.3969/j.issn.1001-1978.2017.11.025
- VernacularTitle:基于UHPLC/Q-TOF/MS分析羊耳菊有效组分的入血成分
- Author:
peng Zi GONG
1
;
ting Ting CHEN
;
yu Jing HOU
;
lin Lin WU
;
Mei LI
;
ting Yue LI
;
ying Si CHEN
;
yu Yan LAN
Author Information
1. 贵州医科大学贵州省药物制剂重点实验室
- Keywords:
Inula cappa;
plasma;
effective fraction;
UHPLC/Q-TOF/MS;
serum pharmacochemistry;
metabolites
- From:
Chinese Pharmacological Bulletin
2017;33(11):1605-1610
- CountryChina
- Language:Chinese
-
Abstract:
Aim To analyze the main components of effective fractions of Inula cappa in rat plasma with UHPLC/Q-TOF/MS technology and serum pharmacochemistry theory.Methods After gavage administration with Inula cappa extracts,blood was collected from abdominal aorta,and the protein in plasma sample was precipitated by methanol.Eclipse Plus C18 RRHD (2.1 mm × 100 mm,1.8 μm) was used,with 0.1％ formic acid agueous solution (A)-0.1％ formic acid and acetonitrile (B) as the mobile phase for gradient elution,and the flow rate was 0.3 mL ·min-1 Detected at negative ion mode,and with the help of Metabolite Tools software from Bruker Corporation,components in plasma were defined.Results A total of 16 compounds were identified,including 9 prototypes and 7 metabolites.Main metabolites were isomerization,methylation,glucuronidation and recombination of caffeoylquinic acid.Conclusions Therefore,our study has comprehensively expounded Inula cappa extracts' constituents migrating to rat plasma,and provided a reference for further studies for in vivo metabolic process and effective material base of Inula cappa.
