Inhibitory effects of curcumin on vasculogenic mimicry in choroid melanoma cell line OCM-1 and its mechanisms
10.13389/j.cnki.rao.2017.0258
- VernacularTitle:姜黄素对人脉络膜黑色素瘤细胞株血管形成拟态抑制作用及其相关机制的研究
- Author:
Wen GAO
1
;
Xia Lu CHEN
;
Jin Yan HE
;
Ming Feng LIANG
Author Information
1. 天津中医药大学第一附属医院眼科
- Keywords:
Choroid melanoma;
curcumin;
vasculogenic mimicry;
PI3K
- From:
Recent Advances in Ophthalmology
2017;37(11):1018-1021
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of curcumin (Cur) on vasculogenic mimicry (VM) and PI3K pathway in choroid melanoma cell line OCM-1 in vitro 3-dimentional culture.Methods Three-dimensional culture of OCM-1 cells was performed for the observation of VM formation and the influence of concentration gradient (0μmol · L-1,10 μmol · L-1,20 μmol · L-1,30 μmol · L-1,40 μmol · L-1) Cur on VM,and immunohis to chemistry staining was used to detect the expression of PI3K and EphA2 in OCM-1 cell line.Results Cur could markedly inhibit the formation of VM in choroid melanoma cell line OCM-1 in vitro 3-dimensional culture with a dose-dependent manner.Cur suppressed EphA2 and PI3K expression in a concentration-dependent manner,and the difference between groups with 0 μmol · L-1 Cur group was significant statistically (all P =0.000),but there was no significant difference between 30 μmol · L-1and40 μmol · L-1 group (EphA2,P=0.300;PI3K,P=0.188).Conclusion Cur can significantly inhibit VM formation in choroid melanoma cell line OCM-1,of which the mechanism may be related to the downregulation expression of EphA2 and PI3K,thus inhibiting PI3K signaling pathway.
