Effect of E.coli K5 polysaccharide on retinal neovascularization in mice with diabetic retinopathy
10.13389/j.cnki.rao.2017.0261
- VernacularTitle:大肠杆菌K5多糖对糖尿病视网膜病变小鼠视网膜新生血管形成作用的研究
- Author:
Fang Lin SONG
1
Author Information
1. 安阳市眼科医院
- Keywords:
K5 polysaccharide;
diabetic retinopathy;
neovascularization generation
- From:
Recent Advances in Ophthalmology
2017;37(11):1032-1035
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of E.coli K5 polysaccharide on retinal neovascularization in mice of diabetic retinopathy.Methods E.coli K5 strain was cultivated by fermentation and the supernatant was used to prepare K5 polysaccharide for analyze its purity by high performance liquid chromatography (HPLC).Moreover,male ICR mice were divided into normal group,model group and K5 polysaccharide treatment group (treatment group for short).The mice in the normal group were healthy and left untreated,while the mice in the model group and treatment group were administrated with streptozotocin to establish diabetic model,and the treatment group received K5 polysaccharide 4 weeks after the successful modeling.Then the eyeball tissues in the three groups were collected 8 weeks after modeling for HE staining and ADPase staining,and real-time PCR and Western blot were used to detect the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) in eyeball tissues.Results HPLC resuits showed that the purity of K5 polysaccharide exceeded 95％.HE staining and ADPase staining suggested that a few of new vessels were observed,and the number of vessels in the model group was significantly higher than those in the normal group,while the treatment group was significantly lower than the model group (P ＜ 0.05).Real-time and Western blot showed that the expression of VEGF and MMP-2 mRNA and protein in the model group were significantly higher than those in the normal group,while the treatment group was significantly lower than the model group (P ＜ 0.05).Conclusion E.coli K5 polysaccharide can inhibit the retinal neovascularization generation in mice with diabetic retinopathy,and the mechanism of its action may be associated with the suppressed expression of VEGF and MMP-2.
