Relationship between Wound Age and the Expression of Fzd2 in Rats Skeletal Muscle after Contusion
10.3969/j.issn.1004-5619.2017.04.002
- VernacularTitle:大鼠骨骼肌挫伤后Fzd2表达与损伤时间的关系
- Author:
na Ta DONG
1
;
Ming PEI
;
Na LI
;
yan Xi ZHU
;
hong Jun SUN
Author Information
1. 山西医科大学法医学院,山西太原030001
- Keywords:
forensic pathology;
wound age estimation;
frizzled-2;
skeletal muscle contusion
- From:
Journal of Forensic Medicine
2017;33(4):344-347
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between wound age and the expressions of frizzled-2 (Fzd2) mRNA and its protein in rats skeletal muscle after contusion,and to explore its possibility of being an index for wound age estimation.Methods The mRNA and protein expressions of Fzd2 in rats skeletal muscle of the control group and the experimental group within 4-48 h after contusion were detected per 4 h by RT-qPCR and Western blotting.Results The relative expression of Fzd2 mRNA increased at 24h,36h and 40h after contusion,and the expression at 24h was twice as the control group (P＜0.05).The relative expression of Fzd2 protein changed inconspicuously after contusion (P＞0.05).Conclusion The changes of Fzd2 mRNA expression after contusion in a certain time can be a basis to estimate wound age by combined multi-indicators.
