Mechanism of Oridonin-induced Apoptosis of Glioma SHG44 Cells
10.12007/j.issn.0258-4646.2017.06.007
- VernacularTitle:冬凌草甲素诱导胶质瘤SHG44细胞凋亡及其分子机制的研究
- Author:
Mang ZHU
1
;
Qianfa LONG
;
Yanping YANG
;
Xinxin FAN
;
Jiangtao XIE
;
Haitao JIANG
Author Information
1. 西安市第三医院神经外科
- Keywords:
oridonin;
glioma;
SHG44 cells;
cell apoptosis
- From:
Journal of China Medical University
2017;46(7):604-608
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular mechanism of oridonin-induced apoptosis of ghoma SHG44 cells.Methods A growth curve was plotted using CCK-8 colorimetric method with different concentrations of oridonin (0,1.25,2.5,5,10,20,and 40 μmol/L)to observe its effect on the growth of SHG44 cells.Hoechst33258 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were used to examine the changes in cell morphology and flow cytometry was used to detect cell apoptosis.Western blotting was used to analyze the expression of apoptosis-related proteins (Caspase-3,cleaved Caspase-3,Bax,and Bcl-2)in SHG44 cells.Results SHG44 cell proliferation was significantly suppressed after 24 and 48 h Oridonin treatment,with a half-maximal inhibitory concentration of 7.865 and 4.74 μmol/L,respectively.Hoechst33258 and TUNEL staining showed changes in cell morphology such as shrinkage and nucleus fragmentation and morphogenesis,which are indicative of apoptosis.Western blotting analysis showed that oridonin inhibited the expression of Bcl-2 and activated the expression of Caspase-3 and Bax.Conclusion Oridonin can inhibit the proliferation and induce the apoptosis of SHG44 cells by regulating the expression of apoptosis-related proteins.
