Effects of Sishen Pills on Ghrelin-CaM-MLCK Signaling Pathway in Sinuses Ventriculi of Rats with Diarrhea of Yang Deficieney of Spleen and Kidney
10.3969/j.issn.1005-5304.2017.12.009
- VernacularTitle:四神丸对脾肾阳虚泄泻大鼠胃窦组织Ghrelin-CaM-MLCK信号通路的影响
- Author:
yuan Xiao LIN
1
;
Na DENG
;
Ying CAI
;
Fang LIU
Author Information
1. 湖南中医药大学第一附属医院
- Keywords:
Sishen Pills;
diarrhea;
Ghrelin;
GHSR;
CaM;
MLCK;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(12):34-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Sishen Pills on Ghrelin-CaM-MLCK signaling pathway with sinuses ventriculi in diarrhea rats of yang deficieney of spleen and kidney; To discuss its mechanism of action. Methods A model rats with diarrhea model of yang deficieney of spleen and kidney was established by treated with intragastric administration of adenine and Senna water decoction to replicate. 72 SD rats were randomly divided into normal group, model group, positive medicine group, Sishen Pills high-, medium- and low-dose groups, with 12 rats in each group. Each medication group was given relevant medicine for gavage, for 14 days. Immunohistochemistry and Western blot were used to detect the expressions of Ghrelin and GHSR, CaM, and MLCK protein in the sinuses ventriculi. Results In the model group, Ghrelin, GHSR, CaM and MLCK protein expressions of the sinuses ventriculi significantly increased (P<0.01). In each medication group, protein expression of the indexes was lower than the model group (P<0.05, P<0.01). The Sishen Pills high-dose group was particularly obvious. Conclusion The effect of Sishen Pills on diarrhea model rats of yang deficieney of spleen and kidney may be associated with regulation of index protein expression in Ghrelin-CaM-MLCK signaling pathway.
