Baicalin alleviated rheumatoid arthritis synovitis of SD rats through TLR2-NF-κB pathway
10.3969/j.issn.1001-1978.2017.11.018
- VernacularTitle:黄芩苷经TLR2/NF-κB途径减轻类风湿关节炎大鼠滑膜炎
- Author:
Lin BAI
1
;
xin Yu YANG
;
feng Qiao WAN
;
Ling HUANG
;
zheng Hai DUAN
Author Information
1. 宁夏医科大学基础医学院病原生物学与免疫学系
- Keywords:
baicalin;
rheumatoid arthritis;
TLR2;
MyD88;
NF-κB p65;
HE staining;
rheumatoid arthritis synovial fibroblasts
- From:
Chinese Pharmacological Bulletin
2017;33(11):1569-1573
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the effects of different doses of baicalin on rheumatoid arthritis (RA) in SD rats and explore the possible mechanism.Methods Firstly,the model of RA in SD rats was prepared and the hind foot swelling was measured;HE staining was used to observe the pathological changes of the knee joint synovial tissue;RT-qPCR was adopted to determine the mRNA expressions of TLR2 and MyD88 in synovial tissue;Western blot was used to determine the protein expressions of TLR2,MyD88 and NF-κB p65 aher intragastric administration of different doses of baicalin solution.Results Compared with the model,baicalin (60 and 30 mg · kg-1) could inhibit the proliferation of fibroblasts and inflammatory damages in synovial tissue,significantly cut down mRNA expressions of TLR2 and MyD88 (P ＜ 0.05) and markedly reduce protein expressions of TLR2,MyD88 and NF-κB p65 (P ＜ 0.01).Conclusion Baicalin has good effects on RA,which may be realized by inhibiting the activation of TLR2-NF-κB signaling pathway.
