Free hormone analysis-current status and application of equilibrium dialysis/ultrafiltration-coupled liquid chromatography tandem mass spectrometry
10.3760/cma.j.issn.1009-9158.2017.10.005
- VernacularTitle:游离激素检测现状及液相色谱串联质谱法的临床应用
- Author:
Zhimin CAO
1
;
Wei GUO
;
Kun ZHONG
Author Information
1. 美国纽约州奥尔巴尼市
- Keywords:
Hormones;
Chromatography;
liquid;
Tandem mass spectrometry;
Clinical chemistry tests
- From:
Chinese Journal of Laboratory Medicine
2017;40(10):748-755
- CountryChina
- Language:Chinese
-
Abstract:
Improving the quality of free hormone test results which is called reliability can improve the diagnosis rates of a series of diseases.However,such a need has not yet been met by the commonly used immunoassay-based methods,partially due to existence of lack of assay accuracy and specificity.Advanced mass spectrometry instrumentation in a combination of equilibrium dialysis or ultrafiltration can avoid the shortage of immunological methods,which is the forefront of the development of hormone detection methods. Free hormone analysis using mass spectrometry may also become gradually adapted as routine testing in clinical laboratories.This review is focused at the related information of free hormone,form of free hormone in body circulating, clinical utility of free hormone analysis, methodologies used for analysis, issues or concerns in quality performance of the commonly used methods, key components of equilibrium dialysis/ultrafiltration coupled liquid chromatography tandem mass spectrometry procedure and of method validation.
