Change of PD-L1 in Peripheral Blood Mononuclear Cells of Patients with Primary Biliary Cirrhosis and Its Significance
10.3969/j.issn.1671-7414.2017.05.001
- VernacularTitle:PD-L1在原发性胆汁性肝硬化患者外周血单个核细胞中的变化及意义
- Author:
da Teng LI
1
;
Yan CHEN
;
li Ming GU
;
lan Yuan HUANG
;
wei Wei ZHANG
;
Yi SUN
;
Jie GUO
;
Yun LIU
;
xia Gui XU
;
An-mei CHENG
;
DENG QIAN
Author Information
1. 第二军医大学长海医院,上海200433
- Keywords:
primary biliary cirrhosis;
PD-L1;
TNF-α;
IL-23
- From:
Journal of Modern Laboratory Medicine
2017;32(5):1-3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of PD-L1 in peripheral blood mononuclear cells (PBMC) from patients with primary biliary cirrhosis (PBC) and its significance.Methods Peripheral blood of 23 healthy individuals and 23 patients with PBC diagnosed in Changhai Hospital was collected during February 2013 to December 2015.PBMC was separated by density gradient centrifugation,then cultured.The mRNA expression of PD-L1 in PBMC was examined by real-time fluorescent quantitative PCR (qRT-PCR),the expression of cytokines such as TNF-α,TGF-β,IL-23,IL-17 in medium was examined by enzyme-linked immunosorbent assay (ELISA).Two independent samples' t test was used to compare variables in two groups.Pearson correlative coefficient was used to reveal the relationship between two variables.Results mRNA expression of PD-L1 in experiment and control groups was 0.23±0.08 vs 1.27±0.40 (t=12.23,P＜0.000 1) and there was statistical significance.Pearson correlation analysis revealed that PD-L1 was negatively correlated with IL-23(r=-0.531,P =0.009) with difference statistically.Conclusion PD-L1 might involve in the development of PBC,and could be the potential biomarker for predicting and treating this disease.
