Establishment and Clinical Evaluation of sH2a Subunit from ASGPR in Diagnosis of Hepatic Injury
10.3969/j.issn.1671-7414.2017.05.003
- VernacularTitle:人血清去唾液酸糖蛋白受体(ASGPR)H2亚基(sH2a)酶联免疫检测法的建立及临床应用
- Author:
fang Xu YIN
1
;
sun Yi FAN
;
Qi WANG
;
bin Pin RAO
;
li Li SHI
Author Information
1. 上海交通大学医学院附属苏州九龙医院检验科
- Keywords:
heptical injury;
asialoglycoprotein receptor (ASGPR);
SH2a;
ELISA
- From:
Journal of Modern Laboratory Medicine
2017;32(5):8-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To diagnose the process of hepatic injury,a method of quantitating the concentration of sH2a subunit from asialoglycoprotein receptor (ASGPR) in serum by enzyme-linked immunosorbent assay was established and clinical evaluated.Methods 210 serum samples were collected in Suzhou Kowloon Hospital.Among them,70 subjects with cirrhosis,viral hepatitis and fatty liver disease were as hepatic injury group and 140 subjects of healthy and high fat,hemolysis,jaundice and with autoimmune disease were as control group.The serum sH2a of two group were measured by ELISA kit.The results of sH2a ELISA were analyzed by four table chi-square test and SPSS20.0 software.Results sH2a protein level in liver injury group and control group were 105.92+ 53.41 ng/ml and 69.25+27.45 ng/ml,respectively.The difference between the control group and the liver injury group was statistically significant (F=14.375,t=5.397,P=0.000).The sensitivity and specificity of the sH2a ELISA kit were 68.57％ (95％CI:56.37～79.15％) and 82.86％ (95％CI:75.58％～88.70％),and the total compliance rate was 78.10％ (95％CI:71.88％～83.49％) with KAPPA coefficient:0.510 6 (95％ CI:0.3877～0.6336).Conclusion SH2a serum ELISA kit with positive and negative coincidence rate between SH2a serum level and meet the clinical requirement,which could be used as new marker for diagnosis of heptieal injury related diseases.
