Role of MicroRNA-125b in Human Ovarian Cancer Cell SKOV3 Induced Apoptosis by Puerarin

VernacularTitle:microRNA-125b在葛根素诱导人卵巢癌细胞SKOV3凋亡中的作用研究
Author: Yue ZHANG ¹ ; xia Hai DUAN ; Lei BAO ; shan Qiao FU
Author Information

1. 西安市第四医院妇产科
Keywords: ovarian; puerarin; microRNA-125b; apoptosis
From: Journal of Modern Laboratory Medicine 2017;32(5):13-15,117
CountryChina
Language:Chinese
Abstract: Objective To explore whether puerarin induced apoptosis of SKOV3 ovarian cancer cells by microRNA-125b.Methods Using the qRT-PCR technique to detect the change of microRNA-125b after puerarin pretreated SKOV3.Using RNA interference technology to inhibit microRNA-125b expression in SKOV3 cells.Using Western blot technique to detect apoptosis related proteins after microRNA-125b lower expression.Results Puerarin could significantly inhibit ovarian cancer cell SKOV3 proliferation activity and promote its apoptosis related proteins expression.And puerarin can promote the expression of microRNA-125b.Inhibition of microRNA-125b expression in ovarian cancer cell SKOV3 could reduce apoptosis protein expression in SKOV3 cell.Conclusion MicroRNA-125b was involved in puerarin induced SKOV3 cell apoptosis,and prompt microRNA-125b is key molecular of the drug resistance in SKOV3.