Gene Analysis for the Sudden Death of Hypertrophic Cardiomyopathy by Whole Exome Sequencing

VernacularTitle:全外显子组测序对肥厚型心肌病猝死者的基因分析
Author: chao Chuan XU ¹ ; zhi Yun BAI ; shu Xin XU ; li Guo L(U) ; ping Xiao LAI ; Rui CHEN ; guang Han LIN ; jian Wen KUANG
Author Information

1. 广东医科大学
Keywords: forensic pathology; cardiomyopathy,hypertrophic; death,sudden,cardiac; exome; mutation
From: Journal of Forensic Medicine 2017;33(4):339-343
CountryChina
Language:Chinese
Abstract: Objective To analyze the related pathogenicity gene mutations in a sudden death of hypertrophic cardiomyopathy (HCM) on whole exome level.Methods Whole exome sequencing (WES) was been performed on a sudden death case sample with pathological features of HCM by Illumina(R) Hiseq 2500 platform.Using hgl9 as the reference sequences,the sequencing data were analyzed.Suspicious single nucleotide variants (SNV) were screened,and the conservatism and function were analyzed by the software such as PhyloP,PolyPhen-2,SIFT,etc.Results After screening,a heterozygous mutation C719R was finally identified in the gene MYBPC3 of this case.Conclusion The molecular anatomy on whole exome level by second generation sequencing technology can help to define the molecular mechanism of HCM and provide a new mothed and thought for analysis of death cause.