DNA Extraction from Old Bones by AutoMate ExpressTM System
10.3969/j.issn.1004-5619.2017.04.010
- VernacularTitle:利用AutoMate ExpressTM系统提取陈旧性骨骼DNA
- Author:
Bin LI
1
;
Zheng L(U)
Author Information
1. 福建省公安厅刑事技术总队
- Keywords:
forensic genetics;
bone;
DNA;
AutoMate ExpressTM system
- From:
Journal of Forensic Medicine
2017;33(4):380-382
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for extracting DNA from old bones by AutoMate ExpressTM system.Methods Bones were grinded into powder by freeze-mill.After extraction by AutoMate ExpressTM,DNA were amplified and genotyped by Identifiler(R) Plus and MinFilerTM kits.Results DNA were extracted from 10 old bone samples,which kept in different environments with the postmortem interval from 10 to 20 years,in 3 hours by AutoMate ExpressTM system.Complete STR typing results were obtained from 8 samples.Conclusion AutoMate ExpressTM system can quickly and efficiently extract DNA from old bones,which can be applied in forensic practice.
