A study on the roles and mechanisms of miR-20a in lipopolysaccharide-induced inflammation of A549 cells
10.3760/cma.j.issn.2096-2932.2017.06.015
- VernacularTitle:微小RNA-20 a对脂多糖诱导A549细胞炎症反应的作用及机制研究
- Author:
Hui HONG
1
,
2
;
杭州市第一人民医院新生儿科
;
Xinqiao YU
;
Beibei WANG
;
Yaojun JIANG
;
Yongjian GONG
;
Yuan YANG
;
Yang YANG
;
Xiaoyu ZHOU
;
Xiaoguang ZHOU
;
Feng YANG
Author Information
1. 210008 南京医科大学附属儿童医院新生儿科
2. 杭州市第一人民医院新生儿科
- Keywords:
RNA;
Severe acute respiratory distress syndrome;
Interleukins;
Apoptosis signal regulating kinase 1;
miR-20a
- From:Chinese Journal of Neonatology
2017;32(6):463-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the roles of miR-20a in lipopolysaccharide induced inflammation of A549 cells and the possible mechanisms.Method The miR-20a mimic/inhibitor were transfected into A549 cells, and the cells were stimulated using lipopolysaccharide for 24 h.Interleukin-6 ( IL-6) and IL-8 were detected at mRNA level and protein level using real-time PCR and ELISA method , respectively.Protein expression of apoptosis signal regulating kinase 1 (ASK1)、P38、P-P38、JNK and P-JNK were detected using Western blot. Result Compared to mimic negative control group , the levels of mRNA and protein expression of IL-6 and IL-8 in the mimic group were all significantly decreased ( P<0.05).Compared to inhibitor negative control group , the levels of mRNA and protein expression of IL-6 and IL-8 in the inhibitor group were all significantly increased (P<0.05).The levels of ASK1, P-P38 and P-JNK protein in the mimic group were significantly lower than the mimic negative control group (P<0.05);the level of protein expression of ASK1, P-P38 and P-JNK in the inhibitor group were all higher than the inhibitor negative control group (P<0.05).Conclusion The regulation of ASK1 by miR-20a may play an important role in the inflammation process of acute respiratory distress syndrome .
