Study on the Improvement Effect and Mechanism of Meng Medicine Wuwei Shaji Powder on Smoke-in-duced Lung Inflammation in Mice
10.6039/j.issn.1001-0408.2017.31.24
- VernacularTitle:蒙药五味沙棘散对吸烟致小鼠肺部炎症的改善作用及机制研究
- Author:
Guilan BAO
1
;
Xiuzhi WANG
;
Fengjuan LIANG
;
Jia LIU
;
Zheng LIU
Author Information
1. 内蒙古民族大学药物化学与药理学研究所
- Keywords:
Meng medicine;
Wuwei Shaji powder;
Smoke;
Inflammation;
Mice
- From:
China Pharmacy
2017;28(31):4411-4414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the improvement effect and mechanism of Meng medicine Wuwei Shaji powder(WSP)on smoke-induced lung inflammation injury in mice. METHODS:ICR mice were randomly divided into blank group(normal saline), model group (normal saline) and WSP group (2 g/kg). Mice in model group and WSP group received passive smoking to induce model of lung inflammation injury,and intragastrically administrated relevant medicine when modeling,once a day,for 28 d. Af-ter administration,enzyme-linked immunosorbent assay was used to detect the tumor necrosis factor α(TNF-α),interleukin-1β(IL-1β),IL-6,IL-10 levels in bronchoalveolar lavage fluids(BALF);the pathological changes of lung tissue were observed by op-tical microscope after hematoxylin-eosin staining;Western blot was adopted to detect the protein expressions of extracellular sig-nal-regulated kinase (ERK1/2),phosphorylated ERK1/2 (p-ERK1/2),p38 mitogen-activated protein kinase (p38 MAPK),phos-phorylated p38 MAPK(p-p38 MAPK),nuclear factor kappaB p65(NF-κB p65)and phosphorylated NF-κB p65(p-NF-κB p65)in lung tissue of mice. RESULTS:Compared with blank group,TNF-α,IL-1β,IL-6 levels in BALF in model group were obviously increased (P<0.01);lung tissue showed significant inflammatory lesions;and the protein expressions of p-ERK1/2, p-p38 MAPK,p-NF-κB p65 in lung tissue were obviously increased (P<0.01). Compared with model group,TNF-α,IL-1β levels in BALF in WSP group were obviously decreased(P<0.05);inflammation injury in lung tissue was obviously improved;and protein expressions of p-p38 MAPK and p-NF-κB p65 in lung tissue were obviously decreased (P<0.05). CONCLUSIONS:WSP shows improvement effect on smoke-induced lung inflammation injury in mice,which might be by blocking the p38 MAPK,NF-κB p65 phosphorycation to inhibit the high expression of inflammatory factors as TNF-αand IL-6.
