Effects of Ginsenoside Rg3 on oxidative stress and apoptosis in diabetic kidney disease rats
10.3969/j.issn.1006-6187.2017.06.015
- VernacularTitle:人参皂苷Rg3对糖尿病肾脏疾病大鼠氧化应激和凋亡的影响
- Author:
Jun TANG
1
;
Manhua ZUO
Author Information
1. 445000,湖北省恩施土家族苗族自治州中心医院肾内科
- Keywords:
Ginsenoside Rg3;
Diabetic kidney disease;
Oxidative stress;
Apoptosis
- From:
Chinese Journal of Diabetes
2017;25(10):929-932
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Ginsenoside Rg3 on oxidative stress and apoptosis in rats with diabetic kidney disease (DKD) induced by STZ.Methods DKD model induced by injection of STZ were randomly divided into 4 groups:diabetic kidney disease group(DKD),low,middle,and high dose Rg3 treated group (L-Rg,M-Rg,H-Rg).Normal rats were selected as control group (NC).Then the Rg3 treated groups were daily administrated with Rg3 (2,5,10 mg/kg) for 10 weeks,while the other two groups were given distilled water.Urinary protein was tested after collecting urine for 24 hours.The fasting plasma glucose (FPG),blood urea nitrogen (BUN),creatinine (Scr),and activity of oxidative stress markers such as GSH-Px,MDA,and SOD in serum were measured.The pathological changes of the kidney were evaluated by HE staining.Cell apoptosis was detected by TUNEL method,and expression of apoptosis related proteins Bax and Bcl-2 were detected by Western blotting.Results After Rg3 intervention,the general status and renal function such as BUN and Scr were significantly improved,24 h urinary protein decreased,serum SOD activity was enhanced,while the content of MDA decreased,apoptosis rate and expression of Bax decreased,and Bcl-2 increased.Conclusion Ginsenoside Rg3 has a protective effect on renal injury in diabetic rats,which may be related to the enhancement of the antioxidase activity in serum,and the inhibition of the apoptosis of renal cells.
