Expression and function of long non-coding RNA AK046999 in the development of mouse cerebral cortex
10.3969/j.issn.1001-6325.2017.11.004
- VernacularTitle:长链非编码RNA AK046999在小鼠大脑皮质发育中的表达及功能
- Author:
ning Jia LI
1
;
jie Jun ZHOU
;
Wei LIU
;
zhong Xiao PENG
;
cheng Peng SHU
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室生物化学与分子生物学系
- Keywords:
AK046999;
long non-coding RNA;
spatio-temporal expression;
cerebral cortex development
- From:
Basic & Clinical Medicine
2017;37(11):1513-1518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression and the role of the long non -coding RNA AK046999 in the development of mouse cerebral cortex.Methods 1) The AK046999 locus was obtained from the UCSC genome browser ,and the coding ability of protein was predicted by Coding Potential Calculator ; 2) The expression profile of AK046999 in the development of mouse cerebral cortex was analyzed by immunofluorescence and in situ hybridization;3)The AK046999 knockout mouse was constructed by TALEN technique and identified at the level of DNA and RNA; 4)The phenotype of knockout mouse cerebral cortex was analyzed by immunofluorescence and TUNEL assay.Results 1)AK046999 had weak coding ability and could be considered as non -coding RNA; 2)AK046999 was highly expressed in VZ /SVZ of the development of mouse cerebral cortex; 3)The AK046999 knockout mouse was successfully constructed; 4) Immunofluorescence results showed that there were no obvious changes in the markers of PAX6, TBR2 and NEUROD2 compared with the control, and apoptotic cells also had no obvious change.Conclusions The AK046999 is expressed in the cerebral cortex of developing mouse and AK046999 knockout has no effect on proliferation, differentiation and apoptosis of neural progenitor cells in the cerebral cortex .
