Pinocembrin alleviates hypoxia reoxygenation injury in rat liver cell line BRL-3A
10.3969/j.issn.1001-6325.2017.11.008
- VernacularTitle:匹诺塞林缓解大鼠肝细胞系BRL-3A低氧/复氧损伤
- Author:
Zhen GUO
1
;
Tong MOU
;
ting Ting LI
;
Di ZHU
;
liang Jun PU
;
jun Zhong WU
Author Information
1. 重庆医科大学附属第一医院肝胆外科
- Keywords:
pinocembrin;
hypoxia/reoxygenation;
hepatocyte injury;
toll-like recepcor4;
nuclear factor-κb P65
- From:
Basic & Clinical Medicine
2017;37(11):1535-1540
- CountryChina
- Language:Chinese
-
Abstract:
Objcetive To investigate protective effect of pinocembrin ( PIN)on hepatocytes induced by hypoxia/reoxygenation ( H/R) as well as its relationship to the TLR 4/NF-κB signaling pathway .Methods The cells were randomly divided into 4 groups: control group, PIN group, hypoxia/reoxygenation injury group and PIN pretreatment group .The cell viability was measured with CCK-8.The apoptosis rate was determined by Annexin V-FITC/PI staining.The activity of ALT was detected .ELISA was used to evaluate the contents of TNF-αand IL-β.The mRNA and protein expression level of TLR 4, IκB-αand NF-κB P65 in cells was observed by quantita-tive real-time PCR or Western blot .Results The H/R stimulation decreased cell survival rate and enhanced the apoptosis .The activity of ALT was increased .The contents of TNF-αand IL-1βwere significantly enhanced , and the expression level of TLR4 and NF-κB P65 was markedly increased while IκB-αdecreased.After pretreatment with PIN, the cell survival rate increased while the apoptosis rate decreased .The activity of ALT was decreased. TNF-αand IL-1βwere reduced significantly and the expression level of TLR 4 and NF-κB P65 were decreased while IκB-αincreased.Conclusions PIN has protective effects on hypoxia/reoxygenation injury, which might be mediated in part by TLR4/NF-κB signaling pathway .
