Screening and Enzymatic Activity Analysis of Dominant Fermentive Bacteria of Sojae Semen Praeparatum from Different Production Places
10.6039/j.issn.1001-0408.2017.31.10
- VernacularTitle:不同产地淡豆豉优势发酵菌群的筛选及酶活性分析
- Author:
Liyan CHEN
1
;
Qing LIU
;
Yinling SUN
;
Ping WANG
;
Lei ZHANG
;
Weiming WANG
Author Information
1. 黑龙江省中医药科学院
- Keywords:
Sojae semen praeparatum;
Production place;
Selective medium;
Dominant bacteria;
Enzyme activity
- From:
China Pharmacy
2017;28(31):4359-4361
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To provide reference for the standardized production of Sojae semen praeparatum (SSP). METH-ODS:SSP samples from Heilongjiang,Hebei,Gansu,Shandong,Anhui and Yunnan were respectively collected. The fermentive bacteria were cultured with the selective medium contained artemisiae annuae herba and mori folium. Foline-phenol method,fibrous protein plate method and p-nitrophenol-β-D-glucoside colorimetric method were respectively conducted to determine the activities of protease,plasmin and β-glucosidase of the strains to screen dominant fermentive bacteria. RESULTS:Totally 14 wild strains were separated from SSP samples from 6 production places, including 3 strains of bacteria and 11 strains of molds. 1 strain of rod-shaped bacteria and 1 strain of Mucor sp. were separated from SSP from Heilongjiang;2 strains of Mucor sp. and 1 strain of rod-shaped bacteria were separated from SSP from Hebei;1 strain of Mucor sp.,1 strain of Penicillium sp.,1 strain of Streptococ-cus sp. and 1 strain of Aspergillus sp. were separated from SSP from Gansu;2 strains of Mucor sp. were separated from SSP from Shandong;1 strain of Mucor sp. and 1 strain of Aspergillus sp. were separated from SSP from Anhui;and only 1 strain of Mucor sp. was separated from SSP from Yunnan. According to the strains category and enzyme activities,No.1 bacillus,No.9 Aspergillus sp.,No.11 and No.14 Mucor sp. were preliminary authenticated as dominant fermentation microorganism,total enzyme activities of the 4 strains were 22.77,25.49,41.32,39.13 U/g respectively. CONCLUSIONS:The fermentive bacteria of SSP from different pro-duction places were different,and the dominant one can be screened preliminary through enzyme activity analysis.
