Down-regulation of COX-2 expression inhibits proliferation and promotes apoptosis in human colon cancer cell line HT-29
10.3969/j.issn.1001-6325.2017.11.014
- VernacularTitle:COX-2表达下调抑制人结肠癌细胞系HT-29增殖并促进凋亡
- Author:
yun Hai LIU
1
;
Rui ZHONG
;
Chen XIE
;
Xia WANG
;
jian Zhi HE
Author Information
1. 江西中医药大学基础医学院
- Keywords:
cyclooxygenase-2 ( COX-2);
colorectal cancer;
HT-29;
biological behavior
- From:
Basic & Clinical Medicine
2017;37(11):1568-1573
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effect of cyclooxygenase-2 ( COX-2) expression on cell viability , proliferation, apoptosis and other biological behavior of human colon cancer cell line HT-29.Methods The expression of COX-2 was examed by Western blot and real-time PCR after siRNA COX-2, empty body was transferred into human color-ectal cancer cell line HT-29 by liposome .Cell viability was measured by CCK-8 method .Cell apoptosis and cell cy-cle was measured by flow cytometry .Cell migration and invasion was detected by scratch test and Transwell method , respectively.The expression of Bcl-2, Bax, matrix metalloproteinase 2 (MMP2) was measured by real-time PCR. The level of protein kinase B ( AKT) phosphorylation was measured by western blot .Results Compared with the control group and empty vector group, the expression of COX-2 protein and mRNA was decreased ( P<0.01) , cell viability was reduced ( P<0.01) , cell apoptotic rate was increased ( P<0.01) , cell cycle was arrested in G phase ( P<0.01 ) , cell migration and invasion ability was decreased ( P<0.01 ) , the expression of Bcl-2, MMP2 mRNA and p-AKT protein was down-regulated (P<0.01), the expression of Bax mRNA was up-regulated in COX-2 inhibition group ( P<0.01 ) .Conclusions Down-regulation of COX-2 expression can significantly inhibit HT-29 cell proliferation , migration and invasion , and induce cell apoptosis , which may be related to the down-reg-ulation of AKT phosphorylation .
