Regulatory Effect of Liuwei Dihuang Pills on Connexin 43 Expression in Hepatocarcinoma CBRH7919 Cells in Vitro

VernacularTitle:六味地黄丸调控肝癌CBRH7919细胞Cx43表达的体外研究
Author: Hua YI ¹ ; Kun WANG ; Dan Yi ZOU ; Qiao LI ; Quan XIA ; Xiu Ting ZHAO ; Yan Biao DU
Author Information

1. 广州中医药大学基础医学院
Keywords: Liuwei Dihuang Pills; serum pharmacology; hepatocarcinoma; gene expression regulation; connexin; cell culture
From: Journal of Guangzhou University of Traditional Chinese Medicine 2017;34(6):872-877
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of Liuwei Dihuang Pills (LDP)on the expression of connexin 43 (Cx43) in rat hepatocarcinoma CBRH7919 cells. Methods LDP-containing serum was prepared by serum pharmacological method. The protein expression of Cx43 in CBRH7919 cells was determined by Western blotting method, indirect immuno-fluorescent staining labeled by fluorescein isothiocyanate (FITC)-laser scanning confocal microscope technology and flow cytometry. And the mRNA expression of Cx43 in the hepatoma cells was detected by reverse transcription real-time quantitative polymerase chain reaction(RT-qPCR). Results Compared with the blank serum control group,the protein and mRNA expression levels of Cx43 were increased by volume fraction of 2.5%, 5%, 10% LDP-containing serum (P < 0.05 or P < 0.01). Conclusion LDP play an anti-cancer role through promoting the expression of Cx43 in CBRH7919 cells, altering Cx43 membrane location, and improving the gap junctional intercellular communication(GJIC).