Correlation between the lncRNA expression level in peripheral blood mononuclear cell and the severity of major depressive disorder patients
10.3760/cma.j.issn.1674-6554.2017.10.004
- VernacularTitle:抑郁症患者外周血单核细胞长链非编码RNA表达水平与抑郁症状严重程度的关系
- Author:
Gaofeng YAO
1
;
Shuyou ZHANG
;
Wei NIU
;
Mingjun HE
;
Lingming KONG
;
Liyi ZHANG
Author Information
1. 解放军第102医院全军心理疾病防治中心
- Keywords:
Major depressive disorder;
Peripheral blood mononuclear cell;
lncRNA;
Depressive symptom;
RT-PCR
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2017;26(10):883-888
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between the long non-coding RNA (lncRNA) expression in peripheral blood mononuclear cell (PBMC) and depressive symptoms in victims of major depression disorder (MDD).Methods A total of 138 consecutive MDD patients who had not taken antidepressant drugs in the last 3 months or were first-episode patients from May 2014 to February 2015 were enrolled in the present study.The expressions of 9 MDD-associated lncRNAs (TCONS l2 000001212,NONHSAT102891,TCONS_00019174,ENST00000566208,NONHSAG045500,ENST00000591189,ENST00000517573,NONHSAT034045 and NONH-SAT142707) in PBMC were determined by real-time fluorescence quantitative PCR,and the severity of the depressive symptoms were evaluated by 24 item Hamiton depressive Scale (HAMD-24).Results The expression level of TCONS L2 00001212 was significantly positively correlated with hopelessness symptoms (r=0.370,P＜0.01),the expression level of TCONS_00019174 was significantly positively correlated with total depressive symptoms,retardation symptoms and hopelessness symptoms (r=0.286,0.346,0.542,all P＜0.01) and the expression level of NONHSAT142707 was significantly positively correlated with total depressive symptoms and hopelessness symptoms (r=0.253,0.525,P＜0.01).The expression level of TCONS_00019174 and NONHSAT142707 in the higher scores subgroup was significantly lower than those in lower scores subgroup(Z=3.238,2.254,P＜0.05).When TCONS_00019174 entered into the regression equation with the total scores as the independent variable,it explained 19.8％ of the variance of total scores.A ROC curve analysis revealed that TCONS_000191745 had moderate intensity prediction function on the severity of the depressive symptoms (AUC=0.833,P＜0.01).When the cutoff value was 8.352 CT,Youden index was 0.495 in maximum with 88.24％ sensitivity and 89.32％ specificity.Conclusion TCONS_00019174 has a good positive prediction performance on symptom severity in MDD patients.
