Relationship between NLRP3 expression and mitochondrial function during mechanical stretch-induced damage to mouse alveolar epithelial cells
10.3760/cma.j.issn.0254-1416.2017.08.026
- VernacularTitle:机械通气性牵张致小鼠肺泡上皮细胞损伤时NLRP3表达与线粒体功能的关系
- Author:
Yanzhu GONG
1
;
Changping GU
;
Ge LIU
;
Yuelan WANG
Author Information
1. 山东大学附属千佛山医院麻醉科
- Keywords:
Biomechanics;
Epithelial cells;
Pulmonary alveoli;
Mitochondria;
NLR family,pyrin domain-containing 3 protein
- From:
Chinese Journal of Anesthesiology
2017;37(8):1001-1003
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the relationship between the expression of Nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) in nerve tissues and mitochondrial function during mechanical stretch-induced damage to mouse alveolar epithelial cells.Methods Alveolar epithelial type Ⅱ cell line MLE-12 cells were divided into 3 groups (n=13 each) using a random number table:control group (group C),cyclic stretch group (group CS) and cyclic stretch plus NLRP3 inhibitor TAK-242 group (group CS+T).MLE-12 cells underwent 20％ cyclic stretch at a frequency of 0.5 Hz (stretch ∶ intermittence =1 ∶ 1) for 4 h in group CS.In group CS+T,after being incubated for 1 h with 1 μ mol/L TAK-242,MLE-12 cells underwent 20％ cyclic stretch for 4 h,and the parameters were similar to those previously described in group CS.The reactive oxygen species (ROS) content and mitochondrial membrane potential (△ΨM) were measured using chemiluminescence assay.Enzyme-linked immunosorbent assay was used to determine the concentration of interleukin-1 beta (IL-1β) in the supernatant.The expression of NLRP3 in MLE-12 cells was detected using Western blot.Results Compared with group C,the △ΨM of cells was significantly decreased,the ROS content and IL-1β concentration were increased,and the expression of NLRP3 was up-regulated in group CS,and the △ΨM of cells was significantly decreased,the ROS content was increased,and the expression of NLRP3 was up-regulated in group CS+T (P＜0.05).Compared with group CS,the △ΨM of cells was significantly increased,the ROS content and IL-1β concentration were decreased,and the expression of NLRP3 was down-regulated in group CS+T (P＜ 0.05).Conclusion Mechanical stretch induces damage to mitochondria through up-regulating the expression of NLRP3,thus leading to damage to mouse alveolar epithelial cells.
