Determination of Furazolidone and Florfenicol in Soil by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.160870
- VernacularTitle:超高效液相色谱-质谱联用检测 土壤中两种抗生素呋喃唑酮和氟苯尼考
- Author:
Qiong NAN
1
;
Chun Jing TANG
;
Zhu Ruo HE
;
Cheng Yu HU
;
Tao WU
Author Information
1. 南开大学环境科学与工程学院
- Keywords:
Ultra performance liquid chromatography-tandem spectrometry;
Furazolidone;
Florfenicol;
Solid phase extraction
- From:
Chinese Journal of Analytical Chemistry
2017;45(11):1600-1605
- CountryChina
- Language:Chinese
-
Abstract:
A detection method for furazolidone and florfenicol in soil with various environmental matrices was established using ultra performance liquid chromatography-tandem spectrometry (UPLC / MS / MS) technique. Extracting solution of a mixture of phosphate buffer (pH = 3) and acetonitrile (3 : 7, V/ V) was used in this experiment. The extracted water samples were enriched by SAX-HLB solid phase extraction column before the process of nitrogen blowing ( high purity nitrogen). The enriched antibiotics were desalted with 8 mL of methanol. Waters BEH C18(2. 1 × 100 mm) column was used for the sample separation. UPLC / MS / MS was carried out for qualitative and quantitative analysis under multi-reaction monitoring mode. The detection limit of the method was determined by 3 times of signal-to-noise ratio, and the limit of determination of the method was determined by 10 times of signal-to-noise ratio. The results showed that the detection limits of furazolidone and florfenicol were 1. 19 and 0. 41 μg / kg, respectively, and the limits of quantitation of furazolidone and florfenicol were 3. 40 and 1. 37 μg / kg, respectively. Besides, recovery experiment showed that, for the soil samples spiked with 50 μg / L furazolidone and florfenicol, the recoveries were 79% for florfenicol and 92% for furazolidone. Similarly, for the soil samples spiked with 200 μg / L furazolidone and florfenicol, the recoveries were 96% for furazolidone and 86% for florfenicol.
