Bioinformatics Analysis of Gene Arrays in an Early Osteoarthritis Model Induced by Anterior Cruciate Ligament Transection and Partial Medial Meniscectomy
10.3969/j.issn.1000-6710.2017.09.007
- VernacularTitle:前交叉韧带离断联合半月板切除诱发的骨关节炎早期模型相关基因芯片数据的生物信息学分析
- Author:
Jiangyu CAI
1
;
Dandan SHENG
;
Jia JIANG
;
Shiyi CHEN
Author Information
1. 复旦大学附属华山医院运动医学科 上海200040
- Keywords:
osteoarthritis;
differentially expressed genes;
bioinformatics
- From:
Chinese Journal of Sports Medicine
2017;36(9):788-792,787
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes in gene expression and biological process of the osteoarthritis (OA) induced by anterior cruciate ligament (ACL) transection and partial medial meniscectomy,so as to provide bioinformatic basis for further studying the molecular mechanism of OA.Methods The gene chip datasets of a rat model of early 0A induced by ACL transection and partial medial meniscectomy were downloaded from GEO databases (submitted by Appleton,et al.).The differential expression genes (DEGs)were identified,and the Gene ontology(GO) as well as the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses for DEGs were conducted using bioinformatic methods.Results A total of 170 DEGs including 97 up-regulated genes and 73 down-regulated genes were identified.The up-regulated genes were mainly enriched in the extracellular matrix (ECM) and were closely related to the ECM-receptor interaction,while the down-regulated genes were mainly enriched in the biological function of muscle contraction and were linked with the peroxisome proliferators-activated receptor (PPAR) signaling pathway.Conclusion The changes of ECM and muscle contraction play a key role in the occurrence and development of OA.The ECM-receptor interaction and PPAR signaling pathway are strongly associated with OA and worthy of further study.
