Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol
10.11895/j.issn.0253-3820.170300
- VernacularTitle:基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用
- Author:
Qi Qiao DING
1
;
Li LI
;
Tao Wen FAN
;
Nan Ya LYU
;
Hua Jian HU
;
Ping Li YAN
;
Quan Su SONG
Author Information
1. 南京农业大学动物医学院
- Keywords:
Chloramphenicol;
Quantum dot submicrobeads;
Immunochromatographic test strip
- From:
Chinese Journal of Analytical Chemistry
2017;45(11):1686-1693
- CountryChina
- Language:Chinese
-
Abstract:
A fluorescent immunochromatographic test strip based on the quantum dots submicrobeads (QBs) was developed for quantitative detection of chloramphenicol (CAP). In this method, monoclonal antibody of CAP and OBs complex fluorescent probe was first prepared using 1-ethyl-3-( 3-dimethylaminopropyl ) carbodiimide / N-hydroxysuccinimide coupling approach, then complete antigen CAP-HS-BSA was synthesized and sprayed on nitrocellulose membrane as test line (T line). Similarly, goat anti-mouse antibody was sprayed as control line (C line). The time required for the analysis was 15 min, and the limit of detection (LOD) for CAP was 0. 1 μg / L, with a working range of 0. 1 - 100 μg / L. In spiked milk samples, the test strip demonstrated high recoveries in the range from 93. 3% to 97. 9% with relative standard deviations of less than 7% .
