Development of Nano-Polymerase Chain Reaction and Its Application
10.11895/j.issn.0253-3820.170097
- VernacularTitle:纳米基因扩增技术及其应用
- Author:
Ming Fu SANG
1
;
Xin LI
;
Jia LIU
Author Information
1. 哈尔滨工业大学(威海)海洋科学与技术学院
- Keywords:
NanoPCR;
Nanomaterials;
Specificity;
Amplification efficiency;
Hot-start;
Review
- From:
Chinese Journal of Analytical Chemistry
2017;45(11):1745-1753
- CountryChina
- Language:Chinese
-
Abstract:
Polymerase chain reaction (PCR) has become one of the powerful technique since its invention in 1980s. Nevertheless, PCR technique is still frequently impaired by its low specificity, poor sensitivity, false positive results, etc. Recently, nanomaterials including metal nanoparticles, carbon nanomaterials, quantum dots and nano metal oxide have been added into PCR solution to improve both quality and productivity of PCR. The nanoparticles assisted PCR ( NanoPCR) has received considerable attentions due to its unprecedented sensitivity, selectivity and efficiency. In this view, the mainly used nanoparticles in NanoPCR, including gold nanoparticles, quantum dots, carbon nanomaterials, graphene and metallic oxide, was firstly summarized. And then, the possible mechanisms for highly improved sensitivity and selectivity were discussed. Finally, recent applications of NanoPCR were described.
