A successful pregnancy using completely immotile but viable frozen-thawed spermatozoa selected by laser.
10.5653/cerm.2017.44.1.52
- Author:
Huanhua CHEN
1
;
Guixue FENG
;
Bo ZHANG
;
Hong ZHOU
;
Jinhui SHU
;
Xianyou GAN
Author Information
1. Center for Reproductive Medicine, The Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning, China. cestbon269@sina.com
- Publication Type:Case Report
- Keywords:
Cryopreservation;
Immotile spermatozoa;
Intracytoplasmic sperm injections;
Lasers
- MeSH:
Cryopreservation;
Embryonic Structures;
Fertility Preservation;
Fertilization;
Humans;
Male;
Oocytes;
Pregnancy*;
Sperm Injections, Intracytoplasmic;
Spermatozoa*;
Survival Rate
- From:Clinical and Experimental Reproductive Medicine
2017;44(1):52-55
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was performed on the day of oocyte pick-up, and no motile sperm were observed after extending the culture for another 4 hours, while a survival rate of 39.8% was detected using the laser. In all, five mature oocytes were injected, resulting in four cases of normal fertilization (80%) on day 1. Further, two high-quality day 3 embryos were transferred, which resulted in a singleton pregnancy. Our study demonstrates that completely immotile spermatozoa are worth cryopreserving for further intracytoplasmic sperm injection, which provides a new insight into male fertility preservation in cases of completely immotile spermatozoa.
