Identify normal and abnormal semen via mRNA fluorescence multiplex amplification system: A preliminary study
10.03618/j.issn.1001-5728.2017.05.004
- VernacularTitle:mRNA荧光复合扩增系统鉴别正常和异常精液初探
- Author:
Chong WANG
1
;
Hemiao ZHAO
;
Qingluan LIN
;
Lan HU
;
Jingjing CHANG
;
Zheng TU
Author Information
1. 公安部物证鉴定中心 法医遗传学公安部重点实验室
- Keywords:
forensic biological evidence;
mRNA;
body fluid stains;
seminal fluid;
azoospermtism
- From:
Chinese Journal of Forensic Medicine
2017;32(5):453-456,461
- CountryChina
- Language:Chinese
-
Abstract:
Objective Construct a mRNA multiplex amplification system to identify different types of semen stains. Methods First, collect normal, oligozoospermia and azoospermia semen samples to make semen stains. Second, extract total RNA with Qiagen RNeasy Micro Kit. Then use reverse transcript PCR to amplify goal mRNA markers: 2 markers for sperm(PRM1, PRM2), 2 markers for seminal plasma(TGM4, SEMG1) and 2 housekeeping genes(TEF, UCE). Results All semen mRNA markers can be detected in normal semen samples. The RFU of sperm mRNA markers are lower in oligozoospermia semen samples than that in normal controls. No sperm mRNA markers can be detected in the azoospermia semen samples, only seminal plasma specific can be detected. Conclusion The differentiation of normal and azoospermia semen can be achieved by using multiplex mRNA fluorescence amplification system. While normal semen and oligozoospermia semen compared to no statistical difference.
